Journal: Nature Communications

Article Title: Cell type-dependent differential activation of ERK by oncogenic KRAS in colon cancer and intestinal epithelium

doi: 10.1038/s41467-019-10954-y

Figure Lengend Snippet: Differential effects of BRAF V600E or KRAS G12V on gene expression and intestinal cell hierarchies. All panels: t-SNE visualisations and clustering of organoid single-cell transcriptomes clustered with k-means, 24 h after induction of FLUC control, BRAF V600E or KRAS G12V transgenes. a Colour code for six k-means clusters, and inferred differentiation trajectories starting at cluster 1 shown as grey overlay. b Colour code for transgene and CD44 positivity, as inferred from flow cytometry. CD44 positivity was used to direct cell selection, and thus relative fractions of CD44-high and -low cells are not representative. For CD44 status of the cell populations, see Supplementary Fig. . c Mapping of cell- and pathway-specific differentiation signatures. Numbers of signature genes detected are given per single-cell transcriptome

Article Snippet: Single-cell RNA-sequencing data were pre-processed using the BD Precise Whole Transcriptome Assay Analysis Pipeline v2.0 .

Techniques: Expressing, Flow Cytometry, Selection